Phytochemical Screening and Spectral Analysis of a Bioactive Compound from the Fraction of Bacopa monniera (L)

Abstract

The present paper highlights Phytochemical study and Spectral Analysis of Bacopa monniera whole plants which was collected, identified, shade dried, authenticated, and powdered for the isolation of extract by soxhlation apparatus, then obtained extract was further purified by thin layer, column chromatography and purified fraction was sent to SAIF, CDRI Lucknow (UP) for spectral analysis (IR, UV, HNMR, CNMR and Mass). As a result of spectral analysis, a compound Bacopasaponin was isolated and confirmed in the biologically active fraction of Bacopa monniera extract. Maximum percentage yield was observed in methanol (9.54%) followed by water 3.56% in the results. In preliminary phytochemical screening, various tests were applied and aqueous and methanolic extract was found to be strong positive for saponin and sapogenin. In Column chromatography of Bacopa monniera methanolic extract, solvent systems viz. Toluene: n-hexane: Ethyl acetate (8:1:1), Toluene: Ethyl acetate: Methanol (3:4:3), Toluene: Ethyl acetate: Methanol: Glacial acetic acid (2:4:3:1), n-Butanol: Distilled water (5:5) and Methanol: distilled water (5:5) were applied and Bm-2 fraction was found to be biologically active. In Thin layer chromatography of Bacopa monniera methanolic extract solvent system viz. Toluene: Ethyl acetate: n-Hexane (8:1:1) was used and Rf value 0.68 was found to be of Bm-2 fraction of Bacopa monneira extract.